Design and fabrication of a microfluidic chip for oocyte denudation.

نوع مقاله : علمی-پژوهشی

نویسندگان

1 Biomedical Engineering Department, Faculty of Technical and Engineering South Tehran Branch, Islamic Azad University, Tehran, Iran.

2 Electrical Engineering Department, Faculty of Technical and Engineering South Tehran Branch, Islamic Azad University, Tehran, Iran

3 Microsystem Fabrication Laboratory, Faculty of Electrical and Computer Engineering, University of Tabriz, Tabriz, Iran.

4 Department of biology, Islamic Azad University, Tabriz Branch, Tabriz, Iran.

چکیده

Intracytoplasmic sperm injection (ICSI) is one of the most successful techniques of Assisted Reproductive Technology (ART) and is mostly in use for the treatment of infertility with male factors. In this method, before injecting sperm into the intracytoplasmic of the oocyte, cumulus cells around the oocyte must be stripped to facilitate the injection process. To achieve this, both enzymatic and mechanical methods are used in embryological laboratories for denudation, which has major deficiencies, including the possibility of damaging the oocyte prior to the injection process. In this research, a microfluidic-based device is introduced for the separation of cumulus cells around the oocyte with minimum manual operations. The results prove high efficiency, and non-destructive denudation of the oocyte with the reduced amount of culture medium leads to the low-cost preparation process of oocytes. The process can also be integrated with ICSI chips under development and will be reported shortly.

کلیدواژه‌ها

عنوان مقاله [English]

Design and fabrication of a microfluidic chip for oocyte denudation.

نویسندگان [English]

  • F. Safaefar 1
  • J. Karamdel 2
  • H. Veladi 3 1
  • M. Fallah Tafti 4
1 SociBiomedical Engineering Department, Faculty of Technical and Engineering South Tehran Branch, Islamic Azad University, Tehran, Iran. al Security Organization
2 ElElectrical Engineering Department, Faculty of Technical and Engineering South Tehran Branch, Islamic Azad University, Tehran, Iranectronics Department, Faculty of Engineering, Islamic Azad University, South Tehran Branch
3 Microsystem Fabrication Laboratory, Faculty of Electrical and Computer Engineering, University of Tabriz, Tabriz, Iran. |Biomedical Engineering Department, Faculty of Technical and Engineering South Tehran Branch, Islamic Azad University, Tehran, Iran.
4 Department of biology, Islamic Azad University, Tabriz Branch, Tabriz, Iran.
چکیده [English]

Intracytoplasmic sperm injection (ICSI) is one of the most successful techniques of Assisted Reproductive Technology (ART) and is mostly in use for the treatment of infertility with male factors. In this method, before injecting sperm into the intracytoplasmic of the oocyte, cumulus cells around the oocyte must be stripped to facilitate the injection process. To achieve this, both enzymatic and mechanical methods are used in embryological laboratories for denudation, which has major deficiencies, including the possibility of damaging the oocyte prior to the injection process. In this research, a microfluidic-based device is introduced for the separation of cumulus cells around the oocyte with minimum manual operations. The results prove high efficiency, and non-destructive denudation of the oocyte with the reduced amount of culture medium leads to the low-cost preparation process of oocytes. The process can also be integrated with ICSI chips under development and will be reported shortly.

کلیدواژه‌ها [English]

  • Assisted Reproductive Technology (ART)
  • Denudation
  • In Vitro Maturation (IVM)
  • Microfluidics
  • lab-on-chip

مراجع

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